![imagej pseudocolor imagej pseudocolor](https://image3.slideserve.com/5456068/3d-surface-plot-of-x-ray-ct-scan-of-the-brain-imagej-aplication-l.jpg)
Check the “ Autoscale ” check box to make viewing images easier. Skip the Bio-Formats instructions and jump to the Pre-processing checks section.īe sure to select Hyperstack in the “View stack with” drop down and Grayscale in the “Color mode” drop down menu. If the Bio-Formats Importer window doesn’t open, don’t worry. Note: Some file types do not require the Bio-Formats Importer. After dragging the file into Fiji, you may see the Bio-Formats Import Options window appear.
IMAGEJ PSEUDOCOLOR HOW TO
If you haven’t read our previous blog article, or forgot how to create the 60X image stack, click here for a refresher. Begin by dragging the 60x Z-stack file of the cellular proliferation biomarker MKI67 in MCF7 cells we took in the last blog article into the ImageJ window.
![imagej pseudocolor imagej pseudocolor](https://static.packt-cdn.com/products/9781783283958/graphics/3958_02_06.jpg)
Opening your Raw Image FilesĪfter starting up ImageJ, you can easily open your raw image files by dragging the file into the ImageJ window. There are also additional resources on how to use ImageJ on the ImageJ Tutorials page ( ). The steps in this article should also be compatible with most other image processing software.įor an in depth introduction to ImageJ and Fiji, please read the Getting Started page ( ). Fiji expands on the standalone ImageJ program to include many useful functions and plugins contributed by the community downloadable at. We will be using the “Fiji Is Just ImageJ” (Fiji) image processing package, which is an open source free distribution package of ImageJ.